NAD+ (nicotinamide adenine dinucleotide), a dinucleotide coenzyme used as a reference compound in in vitro analytical chemistry and enzymatic assay work. 1000 mg vial, lyophilized powder, ≥99% HPLC purity verified by third-party analysis. For research use only.
Research areas
- Analytical chemistry of coenzymes
- In vitro enzymatic assays (NAD+ / NADH couple)
- Analytical characterisation (HPLC, mass spectrometry)
Technical data
| Field | Value |
|---|---|
| Name | NAD+ (nicotinamide adenine dinucleotide) |
| Amount per vial | 1000 mg (≈ 1.51 mmol) |
| Nature | Dinucleotide coenzyme — not a peptide |
| Molecular weight | ≈ 663.4 Da |
| Form | Lyophilized powder (unless stated otherwise) |
| Purity | ≥ 99% (HPLC) — see batch COA |
| Shipped from | France, within 24 business hours |
NAD+, NMN or NR: three compounds, three masses
First point of order: NAD+ is not a peptide. It is a coenzyme — a dinucleotide made of a nicotinamide nucleotide and an adenine nucleotide joined by a pyrophosphate bridge. Peptide vocabulary (sequence, amino acids, denaturation) does not apply; lyophilisation, reconstitution and cold chain do.
Three related compounds are routinely confused. They are not the same molecule, and the price of a vial of one does not compare with the other:
| NR | NMN | NAD+ | |
| Nature | nucleoside | nucleotide | dinucleotide |
| Molar mass | ≈ 255.3 g/mol (base) | ≈ 334.2 g/mol | ≈ 663.4 Da |
| Phosphate groups | 0 | 1 | 2 |
Their molar masses differ by up to a factor of 2.6: they compare in moles, not milligrams. Reference for this vial: 1000 mg of NAD+ = 1.51 mmol, the molar equivalent of roughly 504 mg of NMN or 385 mg of NR as the free base.
This reference is NAD+: the mass measured on the COA should fall around 663 Da.
Why choose NEXUS NAD+
- Third-party verified purity: every batch is HPLC-tested by an independent laboratory, and the certificate of analysis (COA), which reports the measured mass, is available before ordering.
- Verifiable identity: the expected mass (≈ 663 Da) is on the COA — it tells NAD+ apart from NMN (≈ 334) and NR (≈ 255) before you even open the vial.
- Shipped from France: parcel prepared within 24 business hours, delivered in 2 to 5 business days in France and 8 European countries. Free shipping from €120.
- Consistent quality: same supplier, same controls, same packaging from batch to batch.
- Verified reviews: our customers leave their feedback on Trustpilot; we do not edit them.
Reconstitution and handling
The lyophilisate is reconstituted with bacteriostatic water, which contains benzyl alcohol and therefore keeps the solution preserved after opening.
Add the solvent slowly down the vial wall, let it run, then swirl gently: no vigorous shaking, no vortex. The solvent volume never changes the mass in the vial, only the resulting concentration. Our reconstitution calculator gives the concentration for any volume.
Specific to this compound: NAD+ in solution is pH-sensitive, described as stable in mildly acidic conditions and degraded in alkaline media — the opposite of NADH. For an enzymatic assay, a freshly prepared solution remains the reference.
Storage
Lyophilized powder: -20 °C or below, protected from light and moisture; stable for years when unopened. After reconstitution: 2–8 °C, protected from light, with a reference shelf life of 28 days. For longer storage, frozen aliquots are preferable to repeated freeze-thaw cycles.
Common laboratory mistakes
- Treating NAD+ as a peptide. It is a dinucleotide coenzyme: degradation in solution is hydrolysis and pH, not denaturation of an amino-acid chain.
- Comparing NAD+, NMN and NR by mass. Their molar masses differ by up to a factor of 2.6; the comparison only makes sense in moles.
- Preparing the solution in an alkaline buffer. NAD+ is described as degraded in alkaline media, unlike NADH.
- Repeatedly refreezing the solution. Each freeze-thaw cycle degrades a fraction of it. Aliquot as soon as it is reconstituted.
- Leaving the vial open to room air. The lyophilisate is hygroscopic: close it at once and return it to the cold.
Frequently asked questions
Is NAD+ a peptide?
No. It is a coenzyme — a nicotinamide and an adenine nucleotide joined by a pyrophosphate bridge. Peptide vocabulary does not apply, whereas lyophilisation, reconstitution and cold chain do.
Are NAD+, NMN and NR the same product?
No. NR is a nucleoside with no phosphate (≈ 255 g/mol), NMN a nucleotide with one (≈ 334 g/mol), NAD+ a dinucleotide with two (≈ 663 Da). This reference is NAD+, as its mass confirms.
How much NMN does 1000 mg of NAD+ correspond to?
1000 mg of NAD+ is 1.51 mmol, the molar equivalent of roughly 504 mg of NMN or 385 mg of NR as the free base. Comparing these compounds by mass is misleading.
What should I check on the certificate of analysis?
HPLC purity of at least 99% and the measured mass, expected around 663 Da. Each batch's COA is available from the Quality page and on request before ordering.
What should this compound be reconstituted with?
Bacteriostatic water, which contains benzyl alcohol and keeps the solution preserved after opening. Plain sterile water is unsuitable once the vial is pierced more than once. Avoid alkaline buffers.
How long does the reconstituted solution keep?
28 days at 2–8 °C, protected from light. Beyond that, frozen aliquots are preferable to repeated freeze-thaw cycles.
Where is the order shipped from, and how fast?
From France, within 24 business hours. Delivery to a pickup point or home in 2 to 5 business days depending on the country. Free from €120.
In what context may this product be used?
Exclusively for laboratory and in vitro research, by qualified personnel. It is not intended for human or animal consumption, nor for diagnostic or therapeutic use.
NEXUS Quality Standard
Every NEXUS research compound is produced under strict quality control procedures. Each batch undergoes independent analytical testing to verify identity, assay accuracy, purity, microbial safety, endotoxin levels, heavy metal screening, and full batch traceability.
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For laboratory research only. NAD+ 1000mg is not suitable for human or animal consumption. Not a drug, food or cosmetic. To be handled by qualified personnel in accordance with applicable regulations.