BLK Pack — CJC-1295 + Ipamorelin · IGF-1 LR3 · TB-500

An assortment of three research references shipped with handling supplies: one CJC-1295 (without DAC) + Ipamorelin 5 + 5 mg vial, one IGF-1 LR3 1 mg vial, one TB-500 (full-length thymosin β4) 10 mg vial, and two boxes of 10 needles. Three separate reagents, three pathways documented individually. Lyophilized powders, ≥99% HPLC purity verified by third-party analysis. Laboratory research use only.

Research areas

• GHRH / GHS-R axis and GH pulsatility (models)
• IGF-1R signalling in cell culture (PI3K/Akt, MAPK)
• Actin regulation and cell migration (models)

Technical data

What the pack contains, reference by reference

A pack is not a molecule: it is a set of separate vials, each with its own mass, COA and literature.

CJC-1295 without DAC + Ipamorelin — 5 + 5 mg. One vial, two compounds at 5 mg each. CJC-1295 without DAC, or Mod-GRF 1-29 (≈ 3,368 Da), reproduces the first 29 amino acids of human GHRH and binds the Gs-coupled GHRH receptor; Ipamorelin (≈ 711.9 Da) is a selective pentapeptide agonist of the Gq-coupled GHS-R. The vial therefore serves to study two distinct intracellular pathways in the same somatotroph cell. This is the no-DAC form, described as a short stimulus, unlike the DAC form whose published half-life spans days.

IGF-1 LR3 — 1 mg. The Long R3 analogue of human IGF-1: 83 amino acids, ≈ 9,111 Da, against 70 amino acids and ≈ 7,649 Da for native recombinant IGF-1. A thirteen-residue N-terminal extension and a position-3 arginine sharply reduce affinity for the six IGFBP binding proteins, making it a cell-culture tool for IGF-1R signalling. Rarely stated: affinity for IGF-1R itself is not increased — the apparent gain exists only where binding proteins are present.

TB-500 — 10 mg. Full-length thymosin β4, a 43-amino-acid protein, ≈ 4,963 Da, CAS 77591-33-4 — not the short LKKTET fragment (~900 Da) the same trade name sometimes designates; molecular weight settles it. Its best-characterised mechanism is sequestration of monomeric G-actin, which governs cytoskeletal reorganisation and therefore cell migration.

Two boxes of 10 needles. Handling supplies. Bacteriostatic water is not included: see bacteriostatic water.

Why these references are grouped

The three vials do not act on the same lever. CJC-1295 + Ipamorelin sits upstream, on pituitary secretion in neuroendocrine models; IGF-1 LR3 interrogates the receptor directly in culture; TB-500 belongs to another register entirely, the actin cytoskeleton, with no mechanistic link to the first two. Together they cover one experimental question through three independent entry points.

No study covers the combination itself. Everything above was obtained compound by compound, in isolation; no publication evaluates the interactions between these three reagents used together. Pathway complementarity is a catalogue rationale, not a documented result: a pack groups independent reagents, it describes no sequence and implies none.

Reconstitution: volumes to plan for

Each vial is reconstituted separately, with bacteriostatic water, which contains benzyl alcohol and allows multiple draws.

At these volumes each vial gives 20 draws of 10 IU. On the combined vial, concentration is computed per compound: 5 mg each, not 10 mg of one.

The most common trap: the volume of water never changes how much peptide is in the vial. Reconstituting the TB-500 with 5 mL instead of 2 mL gives 2 mg/mL instead of 5 mg/mL — the same total mass in a larger volume; only the graduation changes. Our reconstitution calculator computes any other combination live.

Add the solvent slowly down the vial wall, then swirl gently. No vigorous shaking, no vortex — shear denatures peptides and proteins.

Storage

Lyophilized powder: -20 °C or below, protected from light and moisture; stable for years when unopened. After reconstitution: 2–8 °C, protected from light, with a reference shelf life of 28 days. For longer storage, frozen aliquots are preferable to repeated freeze-thaw cycles.

On a pack, one extra precaution: reconstitute each vial only when it is about to be used, so the others stay lyophilized instead of starting their 28-day window for nothing.

Common laboratory mistakes

• Reconstituting all three vials at once. Every opened vial starts its own 28-day clock, while sealed lyophilizate keeps for years.
• Applying the same water volume to every vial without recalculating. 2 mL gives 0.5 mg/mL on IGF-1 LR3 and 5 mg/mL on TB-500: at the same graduation, a factor of ten separates the masses drawn.
• Computing the combined vial's concentration on its total. CJC-1295 and Ipamorelin are 5 mg each, not 10 mg of one compound.
• Comparing this TB-500 with data obtained on the LKKTET fragment. Two distinct molecules, ~900 Da against ≈ 4,963 Da.
• Using plain sterile water, vortexing, or repeatedly refreezing. Each degrades a fraction of the material.