CJC-1295 without DAC + Ipamorelin — 5 mg + 5 mg

A pairing of a GHRH analogue (CJC-1295 without DAC, also known as Mod-GRF 1-29) and a selective ghrelin-receptor agonist (Ipamorelin). This duo is studied for the synergy between two distinct GH-secretion pathways in neuroendocrine models. 5 mg + 5 mg vial, lyophilized, ≥99% HPLC purity verified by third-party analysis. Laboratory research use only.

Research areas

• GHRH / GHS-R synergy
• GH pulsatility (models)
• Combined secretagogues

Technical data

CJC-1295 with or without DAC: the difference

Both forms share the same base: the first 29 amino acids of human GHRH. DAC is not a dosage — it is a chemical group. The Drug Affinity Complex is a maleimide grafted onto the peptide that binds covalently to serum albumin, protecting it from rapid clearance.

The result is a difference in kinetics, not potency: a half-life described at 5.8 to 8.1 days for the DAC form (Teichman et al., 2006) versus a few tens of minutes without DAC. This reference is the no-DAC version, chosen when a study protocol aims to reproduce a short, near-physiological pulse rather than sustained exposure.

Why pair a GHRH analogue with a secretagogue

CJC-1295 binds the GHRH receptor (Gs-coupled, raising intracellular cAMP); Ipamorelin acts on the growth-hormone secretagogue receptor (GHS-R, Gq-coupled, mobilising the phospholipase C / calcium pathway). The pathways are distinct, and GHRP work also describes attenuation of somatostatin — the physiological brake on GH. In models, combining the two yields a response greater than the sum of either stimulus alone.

Ipamorelin is preferred over older GHRPs for its selectivity: Raun et al. (1998) reported no notable rise in ACTH, cortisol or prolactin at doses well above those producing GH release in animals, unlike GHRP-6.

Reconstitution: a worked example

With 2 mL of bacteriostatic water in a 5 mg + 5 mg vial: 5 mg ÷ 2 mL = 2.5 mg/mL, i.e. 2,500 mcg/mL of each compound. On a U-100 syringe, 10 IU = 0.1 mL = 250 mcg of each compound, giving 20 draws of 10 IU per vial.

The most common trap: the volume of water never changes how much peptide is in the vial. Reconstituting with 1 mL instead of 2 mL gives 5 mg/mL instead of 2.5 mg/mL — the same total mass in half the volume. Only the volume to draw changes. Our reconstitution calculator computes any other combination live.

Add the solvent down the vial wall, then swirl gently. No vigorous shaking, no vortex — shear denatures peptides.

Storage

Lyophilized powder: -20 °C or below, protected from light and moisture; stable for years when unopened. After reconstitution: 2–8 °C, protected from light, with a reference shelf life of 28 days. For longer storage, frozen aliquots are preferable to repeated freeze-thaw cycles.

Common laboratory mistakes

• Confusing DAC with dosage. DAC changes half-life, not how much peptide is present. A "5 mg with DAC" vial and a "5 mg without DAC" vial hold the same mass.
• Using plain sterile water for a multi-draw vial. Without a preservative, the solution is no longer protected once the stopper has been pierced several times.
• Vortexing the vial. Vigorous agitation denatures peptides. Gentle swirling is enough.
• Repeatedly refreezing the reconstituted solution. Each cycle degrades a fraction of the peptide. Aliquot at reconstitution.
• Computing concentration on the vial total. Both compounds are 5 mg each here, not 10 mg of one: concentration is calculated per compound.